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KMID : 1007520140230010225
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Food Science and Biotechnology
2014 Volume.23 No. 1 p.225 ~ p.229
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The Citrus Flavonoid Nobiletin Inhibits Proliferation and Induces Apoptosis in Human Pancreatic Cancer Cells In vitro
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Zhang Yu
Dou Huating
Li Hongjun
He Zhifei
Wu Houjiu
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Abstract
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The cellular effects of nobiletin on humanpancreatic cancer cells (PANC-1) and the mechanisms bywhich nobiletin inhibits the proliferation of these cells wereinvestigated. A MTT assay and flow cytometry were usedto examine cell proliferation and apoptosis, respectively. AWestern blot assay was used to examine expression levelsof the apoptotic proteins bax, bcl-2, and p53. NOB inducedapoptosis in these cells via up-regulation of the proapoptoticprotein bax and down-regulation of the antiapoptoticproteins bcl-2 and p53. The normal cell cycle ofPANC-1 cells was arrested by NOB with a significantincrease in the proportion of G0/G1 phase cells (p<0.05)and a significant decrease in the proportion of S phase cells(p<0.05). NOB can inhibit the proliferation of humanpancreatic carcinoma cells by inducing apoptosis andarresting the cell cycle progression.
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KEYWORD
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nobiletin, pancreatic cancer, cell proliferation, apoptosis, cell cycle
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